Journal: Iranian Journal of Pharmaceutical Research : IJPR

Article Title: In-Vitro Anti-Proliferative and Pro-Apoptotic Properties of Sutureja Khuzestanica on Human Breast Cancer Cell Line (MCF-7) and Its Synergic Effects with Anticancer Drug Vincristine

doi:

Figure Lengend Snippet: Effect of satureja khuzestanica total extract (SKE) on the level of cyclin D1 in MCF-7 cells. Each value represents the mean ± SEM band density ratio for each group. β-actin was used as an internal control. ** P < 0.01 significantly different versus control and vehicle-treated cells

Article Snippet: Primary polyclonal anti-Bax, primary monoclonal anti-Bcl-2 antibodies and primary monoclonal anti-cyclin D1 antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Control

Journal: Iranian Journal of Pharmaceutical Research : IJPR

Article Title: In-Vitro Anti-Proliferative and Pro-Apoptotic Properties of Sutureja Khuzestanica on Human Breast Cancer Cell Line (MCF-7) and Its Synergic Effects with Anticancer Drug Vincristine

doi:

Figure Lengend Snippet: Effect of sub-effective doses of sutureja khuzestanica total extract (SKE, 20 µg/mL) and vincristine (Vin, 150 nM) alone or in combination on the levels of activated caspase 3 (A) and cyclin D1 (B) in MCF-7 cancer cells. β-actin was used as an internal control for loading

Article Snippet: Primary polyclonal anti-Bax, primary monoclonal anti-Bcl-2 antibodies and primary monoclonal anti-cyclin D1 antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Control

Journal: Frontiers in Cellular Neuroscience

Article Title: Leptin-dependent neurotoxicity via induction of apoptosis in adult rat neurogenic cells

doi: 10.3389/fncel.2015.00350

Figure Lengend Snippet: List of primary antibodies and details of immunohistochemical procedures used .

Article Snippet: Membranes were incubated overnight at 4°C with a relevant primary antibody: rabbit monoclonal anti-cyclin D1 (NeoMarker, 1:500), rabbit polyclonal anti-phospho(Thr202/Tyr204)p42/p44 ERK, anti-phospho(Thr308)AKT, or anti-phospho(Tyr705)-STAT3 (Cell Signaling Technology, 1: 1000), then 2 h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1:3000) (Santa Cruz Biotechnology, Paso Robles, CA, USA).

Techniques: Immunohistochemical staining

Journal: Cells

Article Title: Preventive Effect of Fisetin on Follicular Granulosa Cells Senescence via Attenuating Oxidative Stress and Upregulating the Wnt/β-Catenin Signaling Pathway

doi: 10.3390/cells14211704

Figure Lengend Snippet: Effect of fisetin on D-gal-induced impairment of proliferation and cell cycle arrest in SYF-GCs. SYF-GCs were pretreated with fisetin (5 µM, 10 µM, 20 µM, and 40 µM) for 24 h, and the co-cultured with D-gal (200 mM) for another 24 h. ( A – E ) The protein levels of CCND1, CDK2, CDK1, and Cyclin B1 in cultured GCs were analyzed by Western blotting, with β-actin used as a loading control. ( F , G ) Flow cytometric analysis of cell-cycle distribution in GCs following treatments of control, D-gal (200 mM) for senescence, D-gal with fisetin (20 µM) pretreatment, and fisetin alone. ( H , I ) EdU assay in GCs under the indicated treatment. Scale bar: 50 μm. ( J , K ) qRT-PCR analysis of FSHR , StAR , CYP11A1 , LIF , LIFR , COX2 , SP1 , GATA6 , IGFR1 , and INHBA mRNA levels in the cultured GCs. Different lowercase letters indicate significant differences ( p < 0.05).

Article Snippet: Membranes were blocked with 5% non-fat milk for 1 h at room temperature, then incubated overnight at 4 °C with the following primary antibodies: CCND1 (1:500, ER0722, Huabio), CDK2 (1:500, R1309-3, Huabio), CDK1 (1:500, ER31213, Huabio), Cyclin B1 (1:500, R1308-13, Huabio), Caspase-3 (1:500, ER1802-42, Huabio), cleaved Caspase-3 (Asp175) (1:500, 9664, Cell Signaling Technology, Beverly, MA, USA), TOMM20 (1:1000, ET1609-25, Huabio), HO-1 (1:500, ER1802-73, Huabio), NQO1 (1:500, ER1802-85, Huabio), Nrf2 (1:500, ER1706-41, Huabio), phospho-Nrf2 (1:1000, ET1608-28, Huabio), Lamin B1 (1: 1000, Huabio), p53 (1:500, Huabio), CDKN1A/p21 (1:500, HA500156 , Huabio), GSK3 beta (1:500, ET1607-7, Huabio), phospho-GSK3 beta (1:500, ET1607-60, Huabio), β-catenin (1:500, Huabio), Histone H3 (1:500, R1105-1, Huabio), and β-actin (1:5000, EM2001-07, Huabio).

Techniques: Cell Culture, Western Blot, Control, EdU Assay, Quantitative RT-PCR

Journal: Cells

Article Title: Preventive Effect of Fisetin on Follicular Granulosa Cells Senescence via Attenuating Oxidative Stress and Upregulating the Wnt/β-Catenin Signaling Pathway

doi: 10.3390/cells14211704

Figure Lengend Snippet: Expression of β-catenin and cell cycle-related proteins in ovarian and follicular tissues of D280 and D580 laying hens. ( A ) Expression of p53 in ovarian tissues and SYFs from D280 and D580 hens, and ASYFs visualized by IHC. Black arrows represent positive-colored GCs. Scale bar: 100 µm. ( B ) IF staining for β-catenin in the ovaries, SYFs, and ASYFs from D280 and D580 hens. Scale bar: 50 µm. ( C , D ) WB analysis of Lamin B1, β-catenin, p53, and CCND1 expression in granulosa cells of SFYs from D280 and D580 hens. ( E ) The mRNA levels of SIRT1 , p53 , p21 , and p15 were determined by qRT-PCR in GCs isolated from SYFs of D280 and D580 hens. * p < 0.05, ** p < 0.01, *** p < 0.001 represent the differences between SYF-GCs from 280-day-old hens and those from 580-day-old hens.

Article Snippet: Membranes were blocked with 5% non-fat milk for 1 h at room temperature, then incubated overnight at 4 °C with the following primary antibodies: CCND1 (1:500, ER0722, Huabio), CDK2 (1:500, R1309-3, Huabio), CDK1 (1:500, ER31213, Huabio), Cyclin B1 (1:500, R1308-13, Huabio), Caspase-3 (1:500, ER1802-42, Huabio), cleaved Caspase-3 (Asp175) (1:500, 9664, Cell Signaling Technology, Beverly, MA, USA), TOMM20 (1:1000, ET1609-25, Huabio), HO-1 (1:500, ER1802-73, Huabio), NQO1 (1:500, ER1802-85, Huabio), Nrf2 (1:500, ER1706-41, Huabio), phospho-Nrf2 (1:1000, ET1608-28, Huabio), Lamin B1 (1: 1000, Huabio), p53 (1:500, Huabio), CDKN1A/p21 (1:500, HA500156 , Huabio), GSK3 beta (1:500, ET1607-7, Huabio), phospho-GSK3 beta (1:500, ET1607-60, Huabio), β-catenin (1:500, Huabio), Histone H3 (1:500, R1105-1, Huabio), and β-actin (1:5000, EM2001-07, Huabio).

Techniques: Expressing, Staining, Quantitative RT-PCR, Isolation